In your kitchen, you probably have a teaspoon.Įvery teaspoon has about 5 milliliters. But, it is not uncommon for a liquid culture of bacteria to have a billion cells in every milliliter of media. What happens when you need to know how many individual bacterial cells are contaminating a food, living in an environmental sample, or growing in a culture tube? You need some method for counting the bacteria accurately. Huge Micro Problem As you know, bacteria are everywhere, invisible to the naked eye, yet influencing every environment on Earth. The following is a brief explanation of some ways of calculating dilutions that are common in biological science and often used at Quansys Biosciences. There are many ways of expressing concentrations and dilution. The DF for the entire series as a whole is the product of the DF's of each individual dilution. In effect, except for the last dilution, each dilution is both a stock and a working solution. Serial dilutions A sequential set of dilutions in which the stock for each dilution in the series is the working solution from previous dilution.
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